Ehrlich Ascites Tumor Preservation for Fifteen Years—a Simple Method
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چکیده
منابع مشابه
Ehrlich ascites tumor preservation for fifteen years--a simple method.
The Ehrlich ascites tumor was preserved for 15 years by adding to it an equal volume of 40% glycerol and placing the preparation directly into a freezer at -60 C.
متن کاملPreservation of Ehrlich ascites tumor cells with dimethyl sulfoxide.
For a number of years our investigations of Ehrlich ascites tumor (EAT) in Mus musculus as a model for tumor immunity studies have yielded variable LD50 values. To explain these results, the hypothesis that the cell challenge is modified during transfer of the stock cells from animal to animal is being tested. Our first attempts at freezing a stock cell suspension of EAT in the ascitic fluid or...
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of cancer cells may be related to the characteristics of the cell surface membranes (i, 7, 43). For example, Coman and his associates have drawn attention to the importance of such properties of the cell surface as adhesiveness(6, 31) and stickiness (8). Since it is now known that lipids, and in particular phospholipids, are essential components of all cell membranes, including both the plasma ...
متن کاملCholesterol metabolism in the Ehrlich ascites tumor.
account for only about 3% of the cholesterol accumulation in the Ehrlich cells. Since the peritoneal fluid in which the tumor cells grow is rich in very-low-density lipoproteins (VLDL), we wished to determine whether the cholesterol contained in these VLDL might be available for utilization by the cells. During incubation in vitro at 37°,the Ehrlich cells took up large quantities of cholestero...
متن کاملOn Perfused Ehrlich Ascites Tumor Cells
In an attempt to overcome resistance of hypoxic cells to radiotherapy, the combination of a hematoporphyrin derivative (Hpd) and 2-deoxy-D-glu'cose (2-DG), a promising radiomodifier, was evaluated by assessing the alterations in phosphorylated metabolites and bioenergetics induced in perfused Ehrlich :lscites tumor (EAT) cells, using Phosphorus31 Magnetic Resonance Spectroscopy (JiP-MRS). By re...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1974
ISSN: 0003-6919
DOI: 10.1128/am.28.4.726-726.1974